Journal: Nanomaterials
Article Title: Image Tracing of Inflammatory Intestinal Organoids via Computational Clearing
doi: 10.3390/nano16100629
Figure Lengend Snippet: Long-term culture of mIOs derived from crypts of Lgr5-EGFP-IRES-creERT2 mice. ( a ) Schematic of crypt isolation, Matrigel dome seeding, and culture workflow. ( b ) Photograph of mIOs cultured in a 24-well plate. Scale bar, 5 mm. ( c ) The red dashed box in ( b ) is observed by microscopy with 5× objective lens. Scale bar, 400 μm. ( d ) The red dashed box in ( c ) is magnified with 20× objective lens. Scale bar, 200 μm. ( e ) Large-area monitoring of organoid culture. Scale bar, 1 mm. ( f ) Variation in organoid area across passages.
Article Snippet: Lgr5-EGFP-IRES-creERT2 mice (Jackson Laboratory, Bar Harbor, ME, USA) were sacrificed to isolate intestinal crypts.
Techniques: Derivative Assay, Isolation, Cell Culture, Microscopy